Characterization of a protein fraction containing cytochromes b and c1 from mitochondria of Leishmania tarentolae.
نویسندگان
چکیده
A soluble red band fraction was obtained from Leishmania tarentolae cells by sucrose gradient sedimentation of a Triton X-100 lysate. Spectral analysis indicated that cytochrome b was present in the red band: the reduced minus oxidized difference spectra revealed absorption maxima at 562,527, and 431 nm at room temperature and 562, 530, and 422 nm at 77K. In addition, a 28-kDa protein was identified in this fraction which retained heme-associated peroxidase activity even after denaturation on SDS-polyacrylamide gels. The amino acid composition of this protein showed a strong similarity to cytochrome c1 of both bovine and yeast.
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ورودعنوان ژورنال:
- Experimental parasitology
دوره 68 4 شماره
صفحات -
تاریخ انتشار 1989